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影响玉米花药培养效率的因素研究

DOI: 10.3321/j.issn:1000-7091.2001.03.003, PP. 12-16

Keywords: 玉米,花药培养,温度处理,胚诱导率

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Abstract:

对影响玉米花粉胚诱导的因素进行了研究。结果表明:花药接种前对雄穗进行℃低温预处理15~21d效果较好;接种后高温(33℃)连续处理花药,胚出现早、质量好且效率高;活性炭(0.5g/L)加入培养基中能显著地提高胚诱导效率。此外,还对基因型间差异进行了比较分析。

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