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利用类甜蛋白基因诱导表达提高马铃薯对晚疫病的抗性研究

DOI: 10.3321/j.issn:1000-7091.2001.01.013, PP. 67-72

Keywords: 马铃薯晚疫病,类甜蛋白基因,表达,抗病性

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Abstract:

将类甜蛋白基因(TLP)及其紧密连锁的抗除草剂bar基因以农杆菌介导法导入脱毒微型种薯“津引薯8号”,经除草剂筛选后的转基因单株无性繁殖用于分子检测,标记基因bar基因的PCR反应和含TLP基因特异探针的Southern检测结果,转基因植株分别显示出0.54kb和3.0kb的特异性条带,证明TLP基因已成功地整合到转基因马铃薯基因组中。利用TLP基因特异性抗血清对转基因植株进行Western反应检测,结果表明,转基因植株中TLP基因表达量显著高于对照组,经晚疫病菌游离孢子接种离体抗性分析,转基因株系表现出对晚疫病的明显抑制作用和症状发生的延迟作用。

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