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两种凝胶电泳系统对SSR标记多态性的影响

DOI: 10.3321/j.issn:1000-7091.2001.02.009, PP. 43-48

Keywords: 玉米,SSR标记,遗传变异,多态性信息量

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Abstract:

利用35个SSR引物和14个玉米自交系直接比较了3%Metaphor琼脂糖凝胶和12%聚丙烯酰胺凝胶电泳对SSR位点多态性水平的影响。结果表明,两种凝胶系统检测出的等位基因数目和基因多态性水平存在显著差异,12%聚丙烯酰胺凝胶在区分微小差异片段能力上明显高于3%Metaphor琼脂糖凝胶。另一方面在两种电泳检测系统下,14个自交系之间的遗传相似系数相关性(r=0.51)达到了极显著水平(P<0.01)。在10个具有最大多态性信息量的SSR引物中,8个在两种凝胶系统中保持相同。利用8个引物可以对供试自交系进行初步鉴定。笔者认为,3%Metaphor琼脂糖凝胶电泳比较适合在遗传作图或基因定位研究中应用;而在品种指纹图谱分析或遗传多样性研究中,应采用12%聚丙烯酰胺凝胶电泳作为检测工具。

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