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用小孢子培养创建大白菜双单倍体永久作图群体

DOI: 10.3321/j.issn:1000-7091.2003.04.015, PP. 55-57

Keywords: 大白菜(BrassicacampestrisL.ssp.pekinensis),小孢子培养,双单倍体,遗传作图

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Abstract:

用高抗TuMV的高代自交系91-112和高感TuMV的小孢子双单倍体系T12-19杂交的F1植株进行小孢子培养,多次继代培养使小孢子再生苗安全越夏,再经移栽驯化后进行低温处理,定植到日光温室.通过加强田间管理,有效地提高了小孢子培养生产双单倍体的成功率和结实水平,得到一个具有146个白菜双单倍体系的作图群体.

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