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应用PCR方法检测鸡传染性贫血病毒

DOI: 10.3321/j.issn:1000-7091.2003.03.025, PP. 90-92

Keywords: ,传染性贫血病,病毒,聚合酶链式反应

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Abstract:

根据鸡传染性贫血病毒(CIAV)基因组序列,设计一对引物扩增基因组上nt58~nt1042之间684bpDNA片段.在CIAV感染MDCC-MSB1细胞系中提取核酸为模板可扩增出相应DNA片段,且以感染细胞核酸为模板反应敏感性可达1fg,而以其他病原核酸为模板扩增结果均为阴性.应用该PCR方法对实验感染鸡肝脏、脾脏、胸腺、肾脏、法氏囊、骨髓、白细胞、泄殖腔棉拭子等不同样品进行检测,均可扩增出相应DNA片段.证明我们建立的PCR方法敏感性特异性强,可用于临床感染不同组织CIAV的检测.

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