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接种和检测苹果潜隐病毒

DOI: 10.3321/j.issn:1000-7091.2003.03.018, PP. 68-70

Keywords: 试管微嫁接,苹果潜隐病毒,检测

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Abstract:

通过试管微嫁接,分别将苹果褪绿叶斑病毒和苹果茎沟病毒接种到小金海棠组培苗上,并用PAS-ELISA法检测其带毒率.结果表明:接穗小金海棠苹果褪绿叶斑病毒的带毒率为60.0%,其苹果茎沟病毒带毒率为7.%,从而证明用此方法可以对组培苗进行快速病毒接种而得到带毒的实验材料.

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