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小菜蛾对杀螟丹抗性相关序列的代表性差异分析

DOI: 10.3321/j.issn:1000-7091.2003.02.004, PP. 14-16

Keywords: 小菜蛾,杀螟丹,代表性差异分析法

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Abstract:

采用代表性差异分析方法,以小菜蛾的敏感品系作为驱赶扩增子,抗杀螟丹近等基因系作为检测扩增子,通过轮消减杂交得到大小为150~00bp的差异片段,经亚克隆测序并与GenBank等数据库同源比较,发现其中一个亚克隆序列与P450基因有部分同源性,其余亚克隆与已知基因序列的同源性较低,判断这些序列可能是新基因的片段.

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