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小麦转基因再生植株培养体系的优化

DOI: 10.3321/j.issn:1000-7091.2003.01.007, PP. 22-25

Keywords: 小麦,基因型,组织培养体系

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Abstract:

以5个基因型不同的小麦幼胚、幼穗作为供试材料,探讨了品种基因型、ABA处理及生根条件等对小麦离体培养的影响。结果表明,小麦品种豫麦18.64为遗传转化的良好受体材料;0.2mg/LNAA和0.8mg/LIAA可促进幼苗生根;在幼胚愈伤组织诱导前14d,用0.5mg/LABA处理可有效抑制出芽率,并有利于促进胚愈伤组织形成和再生能力的保持。

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