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水稻类病变坏死突变基因的RAPD标记

DOI: 10.3321/j.issn:1000-7091.2003.01.002, PP. 5-8

Keywords: 水稻,类病变坏死突变体,RAPD标记

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Abstract:

中籼3037诱变得到新的类病变坏死突变体Sp1801,以粳稻品种02428为父本与其杂交构建分离群体,并利用RAPD技术对此突变相关基因进行了遗传连锁分析,引物OPI11和OPL03扩增的稳定的差异片段与突变性状紧密连锁,两标记与目的基因的遗传距离分别为0.6cM和5.6cM,并且两差异片段分别为556bp和944bp,因此OPI11-556和OPL03-944可以作为此突变基因的RAPD标记。序列同源性分析表明,OPI11-556与一个抗稻瘟病基因pib有高度同源性。

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