全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

小麦-簇毛麦染色体代换系、易位系Ⅴ染色体RAPD标记筛选

DOI: 10.3321/j.issn:1000-7091.2004.03.026, PP. 103-105

Keywords: 簇毛麦,Pm21基因,小麦,代换系,易位系,抗白粉病基因,RAPD标记

Full-Text   Cite this paper   Add to My Lib

Abstract:

利用105条S系列和100条Operon随机引物,对小麦-簇毛麦代换系(6V/6A)、两个易位系(6VS/6AL,6VS/6DL)及亲本簇毛麦(VV)、硬粒小麦(AABB)、栽培小麦(AABBDD)的多态性进行了筛选分析.80.95%的S系列引物扩增出了结果,且条带较清晰;在100条OP系列引物中均扩增出结果,条带清晰可见.从205个随机引物中发现,只有1个引物OPW03在含有簇毛麦Ⅴ染色体的个材料中均扩增出1条约570bp的谱带,而在栽培小麦和硬粒小麦中没有发现.因此,可以推测这个分子标记(OPW03-570)是位于簇毛麦Ⅴ染色体短臂上的.

References

[1]  Mc Intosh R A. Catalogue of gene symbols for wheat[A].In: Slinkard A E.Proc 9th Iht Wheat Genetics Symp[C]. Saskatoon: Saskatoon University Extension Press,1998.123-127.
[2]  刘金元,陶文静,刘大钧,等.小麦-簇毛麦易位系6VS/6AL中6VS的遗传传递及其所携Pm21基因的遗传稳定性分析[J].植物学报,1999,41(10):1058-1060.
[3]  Liu Z,Sun Q, Ni Z,et al. Development of SCAR markers linked to the Pm21 gene conferring resistance to powdery mildew in common wheat[J].Plant Breeding,1999,118:215-219.
[4]  Zhang Q S, Li Z S. Present status of wheat breeding and related genetic study in ChiNA[J ]. Wheat Inform Science,1993,76:1-15.
[5]  McIntosh R A, Hart G E, Devos K M,et al. Catalogue of gene symbols for wheat[ J ]. Wheat Inform Service,1996,83 (Suppl);47-105.
[6]  齐丽丽,陈佩度,刘大钧,等.小麦白粉病新抗源-基因Pm21[J].作物学报,1995,21(3):257-262.
[7]  Huang X Q, Hsam S L K, Zeller F J. Identification of powdery mildew resistance genes in common wheat( Tritium aestivum L.em Thell. ). Ⅸ.Cultivars, land races and breeding lines grown in ChiNA[ J ].Plant Breed, 1997,116:233-238.
[8]  Chen P D, Qi L L, Zhou B, et al.Development and molecular cytogenetic aNAlysis of wheat-HayNAldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew[ J ]. Theor Appl Genet, 1995,91:1125-1128.
[9]  刘志勇,孙其信,李洪杰,等.小麦抗白粉病基因Pm21的分子鉴定和标记辅助选择[J].遗传学报,1999,26(6):673-682.
[10]  刘守斌,唐朝晖,尤明山,等.簇毛麦染色体组特异性RAPD标记的筛选、定位和应用[J].遗传学报,2002,29(5):453-457.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133