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芥菜植株原位真空渗入遗传转化方法的研究

DOI: 10.3321/j.issn:1000-7091.2004.03.003, PP. 8-12

Keywords: 植株原位真空渗入法,遗传转化,根癌农杆菌,芥菜

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Abstract:

以蕾期的芥菜生态型雪里蕻和圆叶芥为试材,在携带潮霉素磷酸转移酶标记基因的根癌农杆菌菌株GV3101的介导下,对影响芥菜植株原位真空渗入遗传转化效果的真空渗入时间、根癌农杆菌悬浮孢子的浓度、渗入培养基、感染方式以及根癌农杆菌菌株等进行研究.结果表明,真空渗入时间为2min/间歇30s/30s;菌液浓度OD600为0.8;渗入培养基为1/2MS无机+B有机;菌株采用强感染型的;对花蕾进行真空渗入,可以获得最佳转化效果.

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