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中国白菜AFLP分子遗传图谱的构建

DOI: 10.3321/j.issn:1000-7091.2004.01.009, PP. 28-33

Keywords: 大白菜,双单倍体(DH),扩增片段长度多态性(AFLP),遗传图谱构建

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Abstract:

以白菜高抗TuMV白心株系91112和高感TuMV桔红心株系T1291为亲本建立的小孢子DH系作为图谱构建群体,以AFLP标记来构建白菜连锁图谱。通过对64对AFLP引物组合的筛选,利用20对引物得到了263个AFLP多态性位点。经Mapmaker/EXP3.0软件处理,构建了1张含255个标记位点,10个连锁群,覆盖长度为883.7cM的连锁图。255个AFLP位点中偏离孟德尔遗传的比率(Pp>0.05)为150%和275%(Pp>0.01)。

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