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通过基因枪和农杆菌介导用BADH基因转化小麦(英文)

DOI: 10.3321/j.issn:1000-7091.2005.05.001, PP. 1-9

Keywords: 遗传转化,小麦,农杆菌,基因枪,BADH基因

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Abstract:

土壤盐碱是一种严重障碍作物生产的环境因子,甜菜碱醛脱氢酶BADH基因是一种重要的可赋予植物渗透调节抗性的基因。本研究用基因枪法及农杆菌介导法向小麦幼胚和成熟胚愈伤组织导入了BADH基因。用PDS-1000/HE基因枪轰击2933块幼胚愈伤组织,分化出了45株再生植株,分化率为1.53%。PCR分析表明,其中的5株为BADH基因转化植株。用PPT涂抹其叶片,进一步证实了PCR的结果。以小麦成熟胚愈伤组织为受体,用农杆菌介导转化1968块愈伤,仅再生出了5株绿苗,PCR检测结果均为阴性。但对其转化愈伤组织的PCR检测表明,外源基因已在受体细胞中实现了整合。以幼胚愈伤组织为受体,用农杆菌介导转化2933块愈伤,共再生出了21株绿苗。对其进行PCR检测,仅有5株为BADH基因转化植株。转化处理过的幼胚愈伤组织的绿苗再生率(0.72%)高于成熟胚愈伤(0.25%)。与对照相比,所有的转化植株均能够在0.5%NaCl(w/w)条件下正常生长,表明外源BADH基因已经整合并表达。

References

[1]  Patnaik D, Khurana P1 Genetic transformation of Indian bread (T1 aestivum) and pasta (T1 durum) wheat by particle bombardment of mature embryo derived calli [J]. Bio Med Central, 2003, 3(5).
[2]  Miroshnichenko D, Filippov M, Dolgov S1 Genetic transformation of Russian wheat varieties for the biotic and abiotic stress resistance [A]. In: Proceedings of the 4th International Crop Science Congress Brisbane, Australia[C]. 2004.
[3]  Kim J K, Duan X L, Wu R, et al. Molecular and genetic analysis of transgenic rice plants expressing the maize ribosome-inactivating protein b-32 gene and the herbicide resistance bar gene[J]. Molecular Breeding, 1999, 5: 85-941
[4]  Cheng M, Fry J E, Pang S, et al1 Genetic transformation of wheat mediated by Agrobacterium tumefaciens [J]. Plant Physiology, 1997, 115: 971-980.
[5]  Wu H, Sparks C, Amoah B, et al1 Factors influencing successful Agrobacterium mediated transformation of wheat[J].Plant Cell Reports, 2003, 21(7): 658-668.
[6]  Tingay S, McElroy D, Kalla R, et al1 Agrobacterium tumefaciens-mediated barLey transformation[J].The Plant Journal,1997, 11: 1369-1376.
[7]  Ozgen M, Onde S, Birsin M, et al1 Transient expression of beta-glucuronidase (GUS) gene in mature embryos of winter durum wheat via microprojectile bombardment [J]. Wheat Information Service, 1999, 89: 30-32.
[8]  Khanna H K, Daggard G E1 Agrobacterium mediated transformation of wheat using a superbinary vector and polyamine supplemented medium[J]. Plant Cell Reports, 2003, 21(5),429-436.
[9]  Li Y, Jia G, Chen H, et al1 Genetic modification with BADH gene to improve the salt tolerance of tomato[A]. In: Proceedings of the second international conference on sustainable agriculture for food, energy and industry [C]. 2002, 1: 865-877.
[10]  Liang Z, Ma D, Tang L, et al1 Expression of the spinach betaine aldehyde dehydrogenase (BADH) gene in transgenic tobacco plants[J]. China J Biotechnol, 1997, 13 (3): 153-159.
[11]  Yan L, Loukoianov A, Blechl A, et al1 The wheat VRN2gene is a flowering repressor down-regulated by vernalization [J]. Science, 2004, 303: 1640-1644.
[12]  Zhang Y, Ding Z,Wen Z, et al1Betaine accumulation and betaine aldehyde dehydrogenase enzyme activity for transgenic wheat under stress condition[J]. Acta Agriculturae Boreal-i Sinica, 2003, 18 (special issue), 36-391 (in Chinese).
[13]  Abebe, Guenzi T A C, Martin B, et al1 Tolerance of manito-laccumulating transgenicwheat to water stress and salinity[J]. Plant Physiol, 2003, 131: 1748-1755.
[14]  Kumar S, Dhingra A, Daniell H1 Plastid-expressed betaine aldehyde dehydrogenase gene in carrot cultured cells, roots, and leaves confer enhanced salt tolerance[J]. Plant Physiol,2004, 136(1): 2843-2854.
[15]  Guo B, Zhang Y, Li H, et al1 Transformaton of wheat with a gene encoding for betaine aldehyde dehydrogenase (BADH) [J]. Acta Botania Sinica, 2000, 42(3): 279-283.
[16]  Vasil V, Castillo A M, Fromm N E, et al1 Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus [J]. BioTechnology, 1992, 10: 667-674.
[17]  Christou P1 Strategies for variety-independent genetic transformation of important cereals, legumes and woody species utilizing particle bombardment[J]. Euphytica, 1995, 85: 13-27.
[18]  Rasco Gaunt S, Riley A, CannellM, et al1 Procedures allowingthe transformation of a range of European elite wheat varieties via particle bombardment [J]. J Exp Bot, 2001, 52 (37), 865-874.
[19]  Tosi P, R DcOvidio, Napier J A, et al1 Expression of ep-itope-tagged LMW glutenin subunits in starchy endosperm of transgenic wheat and their incorporation into glutenin polymers [J]. Theor Appl Genet, 2004, 108 (3): 468-476.
[20]  Nehra N S, Chibbar R N, Leung N, et al1 K1 Sel-f fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs [J]. Plant Journal, 1994, 5: 285-297.
[21]  Finer J J, McMullenM D1 Transformation of cotton (Gossypium hirsutum) via particle bombardment [J]. Plant Cell Rep, 1990, 8: 586-589.
[22]  Jia G, Zhu Z, Chang F, et al1 Transformation of tomato with the BADH gene from Atriplex improves salt tolerance [J].Plant Cell Rep, 2002, 21: 141-146.
[23]  Zhang G, Lu S, Chen S, et al1 Transformation of triploid Bermuda grass (Cynodon dactylon x C1 transvaalensis, cv1 TifEagle) with BADH gene for drought tolerance [A]. In:Proceedings of the Tenth Anniversary Rutgers Turfgrass Symposium[C]. Rutgers University, 2001.
[24]  Mickelbart M V, Ejeta G, Rhodes D, et al1 Assessing the contribution of glycinebetaine to environmental stress tolerance in sorghum[A]. Workshop onMolecular Approaches for the Genetic Improvement of Cereals for Stable Production in Water-Limited Environments (CIMMYT)1999.

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