全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

蒙古马MSTN基因第三外显子的克隆及其SSCP研究

DOI: 10.3321/j.issn:1000-7091.2005.03.004, PP. 14-16

Keywords: 蒙古马,肌生成抑制素,第三外显子

Full-Text   Cite this paper   Add to My Lib

Abstract:

依据马肌生成抑制素(MSTN)编码序列设计引物,以蒙古马基因组DNA为模板,利用PCR技术,扩增出MSTN基因第三外显子序列。将所得片段与PGEM—Teasy载体连接,转化感受态DH5a大肠杆菌。筛选出阳性克隆,酶切、PCR鉴定后,经DNA测序结果表明,所克隆到的MSTN基因第三外显子与文献报道基本一致,有一处单核苷酸有变异。

References

[1]  Mcpherron A C, Lawler A M, Lees J. Rephhation of skeletal muscle mass in mice by a new TGFβsuperfamily mwmber [J]. Nature, 1997, (387): 83-89.
[2]  阉隆飞,张玉麟.分子生物学[M].北京:中国农业大学出版社,1997.
[3]  Sonstegard T S, Rohrer G A, Smith T P. Myostatim maps to porcine chromosme 15 by linkage and physical analyses[J]. Anim Genet,1998,29(1):19-22.
[4]  Mcpherron A C,Lee S J. GDF-3 and GDF-9: two new members of the transforming growth factorβsuperfamily containing a novel pattern of cysteines [J].Jbiol chem., 1993, (268):3444-3449.
[5]  Mcpherron A C, Lee S J. Double muscling in cattle due to mutations in the myostatin gene [J]. Proc Nat (Acad sciv S A, 1997, 94 (23):12 457-12461.
[6]  陈志芳.生长分化因子-8与骨骼肌再生[J].口腔颌面外科,2002,(1):54-57.
[7]  Kambadur R,Sharmam, Smith T P L. et al. Mutations in myostatin (GDF-8) in Double-Muscled Belgian blue and piedm ontese cattle[J]. Genome Res, 1997,7(9):910-916.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133