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农杆菌介导粳稻高效转化体系的研究

DOI: 10.3321/j.issn:1000-7091.2005.03.003, PP. 10-13

Keywords: 农杆菌,转基因,粳稻

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Abstract:

以5个粳稻品种为材料,研究农杆菌介导转化中影响粳稻转化的几个重要因素。通过研究发现,根癌农杆菌菌株EHA105的转化率明显高于LBA4404,根癌农杆菌菌株EHA105介导的水稻转化率为25.3%,而LBA4404为7.6%;不同外植体的诱导率、转化率和分化率存在明显差异,以幼胚为外植体材料,它的出愈率和转化率明显比成熟胚高,而分化率则明显低于成熟胚;不同水稻品种在转化率与分化率上差异也很明显,反映了不同基因型水稻品种的差异,同时研究发现同一品种不同批次在转化率和再生频率上差异比较显著,反映了在水稻转化过程中还存在一些未知条件对于转化的影响;通过对抗性愈伤和转化植株的叶片、根的GUS染色,证明GUS基因在不同组织中表达;并且通过PCR和Southern杂交分子分析,发现所检测的植株均为转基因植株,足以证明这个高效转化体系的可靠性。

References

[1]  Robert H. Agrobacterium tumefaciens transformation of monocotyledons[J]. Crop Science, 1995,35: 301-309.
[2]  Hiei Y, Ohta S, Kumashiro T, et al. Efficient Transformation of rice mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA[J]. The Plant Journal, 1994,6(2):271-282.
[3]  Stephen A Goff,Darrell Ricke,Tien-Hung Lan,et al. A draft sequence of the rice genome ( Oryza sativa L. ssp.japonica ) [J]. Science, 2002,296: 92-100.
[4]  Feng Qi, Zhang Yujun, Hao Pei, et al. Sequence and analysis of rice chromosome 4 [J]. Nature, 2002,420: 316-320.
[5]  Hiei Y, Komari T, Kubo T. Transformation of rice mediated by Agrobacterium tumefaciens [J]. Plant Molecular Biology, 1997,35: 205-218.
[6]  Jeong Dong-hoon, An Suyoung, Kang Hong-gyu, et al.T-DNA Insertional Mutagenesis for Activation Tagging in Rice [J]. Plant Physiology, 2002,130: 1636-1644.
[7]  Li L C, Qu R D, Be achy R N, et al. An improved rice transformation system using the biolistic method [J].Plant Cell Reports, 1993,12:250-254.
[8]  易自力,曹守云,王力,等.提高农杆菌转化水稻频率的研究[J].遗传学报,2001,28(4):352-359.
[9]  Chan M T, Chang H H, Ho S L, et al. Agrobacterium mediated production of transgenic rice plabts expressing a chimeric a-amylase promoter/b-glucuronidase gene [J].Plant Mol Biol, 1993,22: 491-506.
[10]  Jun Yu, Songnian Hu,Jun Wang, et al. A draft sequence of the rice genome( Oryza sativa L. ssp. indica) [J]. Science, 2002,296: 79-92.
[11]  Sasaki T, Matsumoto T, Yamamoto K, et al. The Genome Sequence and Structure of Rice Chromosome 1[J]. Nature, 2002,420: 312-316.
[12]  Jeon Jong-seong,Lee Sichul, Jung Ki-hong, et al. TDNA insertional mutagenesis for functional genomics in rice[J]. The Plant Journal, 2000,22: 561.
[13]  Jung Ki-hong, Hur Junghe, Ryu Choong-hwan, et al.Characterization of a Rice Chlorophyll-Deficient Mutant Using the T-DNA Gene-Trap System [J]. Plant Cell Physiol,2003,44(5):463-472.
[14]  Lee A Sang-choon, Kima Ji-youn, Kima Sung-hyun, et al. Trapping and characterization of cold-responsivegenes from T-DNA tagging lines in rice [J]. Plant Science,2004,166: 69-79.
[15]  翟文学,李晓兵,田文忠.由农杆菌介导将白叶枯病抗性基因Xa21转入我国的5个水稻品种[J].中国科学(C辑),2000,30(2):200-206.
[16]  王关林,方宏筠.植物基因工程[M].北京:科学出版社,2002.

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