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油菜外植体离体培养的细胞分化研究

DOI: 10.3321/j.issn:1000-7091.2005.02.007, PP. 23-25

Keywords: 油菜,外植体,离体培养

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Abstract:

为了探索油菜外植体分化再生的机理,利用扫描电镜技术对芥菜型油菜DB的下胚轴、子叶在不同的离体培养时期的细胞分化和形态变化进行了研究.结果表明,下胚轴外植体是切面上的维管束薄壁细胞和距离切面400~600μm的薄壁细胞脱分化,然后再分化形成芽.而子叶仅仅是距离切面400~600μm的薄壁细胞脱分化后再分化成芽的.

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