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农杆菌介导的水稻遗传转化与植株再生

DOI: 10.3321/j.issn:1000-7091.2005.02.003, PP. 8-11

Keywords: 水稻,成熟种子,组织培养,有机营养物,遗传转化

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Abstract:

以水稻品种ZH11和RBQ为材料,通过水稻成熟胚盾片诱导有效愈伤组织及农杆菌介导的遗传转化技术,获得水稻转基因植株,为水稻转基因体系的建立和完善奠定基础.结果表明:NMB培养基适合水稻成熟胚盾片愈伤组织诱导,MS培养基适合愈伤分化;500mg/L谷氨酰胺或精氨酸有利于愈伤组织的形成;培养条件:愈伤组织诱导时温度25~28℃,暗培养,pH5.8;分化时温度25~28℃,光照1500~2000lx,12~1h/d,pH5.8;6-BA对继代过程中愈伤组织的生长有促进作用;潮霉素筛选的最佳浓度为50mg/L.

References

[1]  Hiei Y, Ohta S. Efficient transformation of rice (Oryzasativa L. ) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA[J]. Plant J, 1994, 6:271-282.
[2]  Edwards K, Johnstone C, Thompson C. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis[J]. Nucleic Acids Research, 1991, 1(6):1349.
[3]  萨姆布鲁克, 弗里奇.分子克隆(第二版)[M].北京:科学出版社, 1992.672-683.
[4]  叶和春.水稻细胞悬浮培养及再生植株的研究[J].植物学报, 1984, 26(1):52-59.
[5]  张玲, 谢崇华, 李卫锋.水稻成熟胚组织培养研究[J].杂交水稻, 2002, 17(2):44-46.
[6]  Ramesh S, Nagadhara D. Production of transgenic indica rice resistant to yellow stem borer and sap-sucking insects, using super-binary vectors of Agrobacterium tumefaciens[J]. Plant Science, 2004, (166):1077-1085.
[7]  Baba A, Hasezawa S, Syono K. Cultivation of rice protoplasts and their transformation mediated by Agrobacterium spheroplasts[J]. Plant Cell Physiol, 1986, 27:463-468.
[8]  刘巧泉, 张景六, 王宗阳, 等. 根癌农杆菌介导的水稻高效转化系统的建立[J]. 植物生理学报, 1998, 24(3):259-271.
[9]  Diah R, Tzkehiko H.Agrobacterium-mediated Transformation of Javanica Rice cv.Rojolele[J]. Biosci Biotechnol Biochem, 2004, 68(6), 1193-1200.
[10]  Lin Y J, ZHANG Qi-fa.Optimising the tissue culture conditions for high efficiency transformation of indica rice[J]. Plant Cell Reports, 2004, (43):6.
[11]  曹明霞, 卫志明, 黄健秋.根癌农杆菌介导的水稻遗传转化[J].植物生理学通讯, 2002, 38(5):423-427.

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