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钙与钙调素对番茄果实乙烯生物合成和信号转导基因表达的调控

DOI: 10.3321/j.issn:1000-7091.2005.01.001, PP. 1-5

Keywords: 钙调素,,乙烯,基因表达

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Abstract:

为了研究CaM和Ca2+在调控乙烯生物合成和信号转导组分基因表达中的相互关系,利用272mmol/LCaCl2及272mmol/LCaCl2+100μmol/LW5(或W7)处理绿熟期番茄果实圆片,然后采用无菌培养的方法在20℃的条件下进行培养。结果表明:CaCl2处理能够抑制番茄果实圆片的乙烯生成,抑制LeACO1,NR,LeERF2基因的表达,而CaCl2+W5(或W7)能消除Ca2+对果实圆片乙烯生成LeACO1,NR,LeERF2基因的表达的抑制作用,但CaCl2和CaCl2+W5(或W7)处理均对LeACS2基因表达影响不明显。以上研究结果表明外源钙处理对乙烯生物合成和信号转导途径中相关基因表达的影响可能与CaM有关。

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