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与黄瓜抗枯萎病基因连锁的RAPD标记

DOI: 10.3321/j.issn:1000-7091.2006.02.029, PP. 121-123

Keywords: 黄瓜,枯萎病,抗性基因,RAPD

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Abstract:

以黄瓜抗枯萎病亲本WIS2757和感枯萎病亲本津研2号及其F2分离群体为试材,采用分离群体分组分析法(BSA)进行了与黄瓜抗枯萎病基因连锁的分子标记研究。运用RAPD技术,利用780条RAPD引物对抗、感亲本进行筛选,其中有11条引物在两亲本之间表现多态性,但仅有引物S49在两组间多态性标记与亲本的多态性标记相同。经F2单株分析,引物S49扩增出的特异DNA片段与WIS7757抗黄瓜枯萎病基因连锁,遗传距离为14cm。DNA标记条带大约为00bp,定名为S49-00。

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