全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

小麦耐盐相关基因LCT多样性研究

DOI: 10.3321/j.issn:1000-7091.2006.02.002, PP. 6-9

Keywords: 小麦,耐盐性,LCT,多样性

Full-Text   Cite this paper   Add to My Lib

Abstract:

LCT1可能是普通小麦Na+和Cs+吸收的一条重要途径。因此,发掘小麦品种中LCT1自然变异,对于通过常规育种或基因工程提高小麦耐盐性具有重要意义。根据小麦离子转运蛋白LCT1(low-affinitycationtransporter)基因cDNA序列(NCBI登录号AF015523),设计引物对8个小麦品种中LCT多样性进行了初步研究。通过克隆测序和全序列比对在8个小麦品种中发现10种LCT序列,分别命名为LCT2-1,LCT2-2,LCT2-3,LCT3-1,LCT3-2,LCT3-3,LCT3-,LCT3-5,LCT3-6和LCT3-7。结果表明:10种LCT均与LCT1存在差异。LCT2-1,LCT2-3,LCT3-2,LCT3-3,LCT3-,LCT3-6和LCT3-7序列发生碱基缺失,缺失均发生在其氨基端。10种LCT序列单碱基突变既有颠换又有转换且均为有义突变。不同小麦品种含有的LCT种类差别较大,其中茶淀红最多(种),而百农3217和苏麦3号仅发现1种。在6个小麦品种中都发现LCT3-1。

References

[1]  Schachtman D P,Schroeder J I.Structure and transport mechanism of a high-affinity potassium uptake transporter from higher plants[J].Nature,1994,370:655-658
[2]  Uozumi N,Kim E J,Rubio F,et al.The Arabidopsis HKT1 gene homolog mediates inward Na+currents in Xenopus laevis oocytes and Na+uptake in Saccharomyces cerevisiae[J].Plant Physiol,2000,122:1249-1259.
[3]  Horie T,Yoshida K,Nakayama H,et al.Two types of HKT transporters with different properties of Na+and K+transport in Oryza sativa[J].Plant J,2001,27(2):129-138
[4]  Rubio F,Gassmann W,Schroeder J I.Sodium-driven potassium uptake by the plant potassium transporter HKT1 and mutations conferring salt tolerance[J].Science,1995,270,1660-1663.
[5]  Rubio F,Schwartz M,Gassmann W,et al.Genetic selection of mutations in the high affinity K+ transporter HKT1 that define functions of a loop site for reduced Na+ permeability and increased Na+ tolerance[J].J Biol Chem,1999,271 (11):6839-6847.
[6]  Liu W,Schachtman D P,Zhang W.Partial deletion of a loop region in the high affinity K+ transporter HKT1 changes ionic permeability leading to increased salt tolerance[J].J Biol Chem,2000,275:27924-27932.
[7]  Amtmann A,Fischer M,Marsh E L,et al.The wheat cDNA LCT1 generates hypersensitivity to sodium in a salt-Sensitive yeast strain[J].Plant Physiol,2001,126:1061-1071.
[8]  Nesbitt T,Tanksley S D.Comparative sequencing in the genus Lycopersicon:Implications for the evolution of fruit size in the domestication of cultivated tomatoes[J].Genetics,2002,162:365-379.
[9]  Tester M,Davenport R.Na+ tolerance and Na+ transport in higher plants[J].Annals of Botany,2003,91,503-527.
[10]  Schachtman D P,Kumar R,Schroeder J I,et al.Molecular and functional characterization of a novel low-affinity cation transporter (LCT1) in higher plants[J].Proc Natl Acad Sci USA,1997,94:11079-11084
[11]  Diatloff E,Kumar R,Schachtman D P.Site directed mutagenesis reduces the Na+ affinity of HKT1,an Na+ energized high affinity K+ transporter[J].FEBS Lett,1998,432(1-2):31-36.
[12]  Maser P,Hosoo Y,Goshima S,et al.Glycine residues in potassium channel-like selectivity filters determine possium selectivity in four-loop-per subunit HKT transporters from plants[J].Proc Natl Acad Sci USA,2002,99:6428-6433.
[13]  Clemens S,Antosiewicz D M,Ward J M,et al.The plant cDNA LCT1 mediates the uptake of calcium and cadmium in yeast[J].Proc Natl Acad Sci USA,1998,95:12043-12048.
[14]  Peng J,Richards D E,Hartley N M,et al.Green revolution genes encode mutant gibberellin response modulators[J].Nature,1999,400:256-261.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133