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小鼠催乳素释放肽的初步克隆

DOI: 10.7668/hbnxb.2007.06.041, PP. 199-200

Keywords: 小鼠,催乳素释放肽,克隆,序列

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Abstract:

为克隆并分析小鼠催乳素释放肽基因,从小鼠下丘脑提取总RNA,进行RT-PCR,PCR产物与pMD-19T连接后转化E.coliDH5α,检测阳性克隆并测序。测序结果表明,所得序列与大鼠催乳素释放肽编码区序列的相似指数为86.3%。克隆所得序列为小鼠催乳素释放肽的基因片段。

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