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口蹄疫病毒株OA/58L蛋白酶的结构构建和功能分析

DOI: 10.7668/hbnxb.2007.04.040, PP. 172-175

Keywords: 口蹄疫病毒,L蛋白酶,活性位点

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Abstract:

以口蹄疫病毒株OA/58RNA为模板,反转录并扩增目的cDNA,然后与pGEM-TEasy载体连接并转化JM109菌株,提取的重组质粒用凝胶电泳、PCR和EcoRI酶切法鉴定。该毒株与A12,O1K,O1Campos和TW5/97毒株序列通过对比分析发现,L基因中有2个起始密码子,第二个是首选;并且确定氨基酸保守区主要位于第35~39,3~5,65~67,75~80,90~111,113~12,16~16,18~157,159~172和176~187位。L蛋白酶含有球状区域,碳端有一柔性杆状结构。合成的L蛋白酶可形成二聚体结构。第1位的Lys、18位的His和163位的Asp可能是L蛋白酶的活性位点。保守区氨基酸残基在维持蛋白的空间构像和功能方面具有重要作用。由拉马钱德兰图可证明本试验构建L蛋白酶的空间结构是合理的,它对于进一步的研究具有指导性。

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