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牛BMP-15基因cDNA的克隆和序列分析

DOI: 10.7668/hbnxb.2007.04.015, PP. 63-66

Keywords: ,BMP-15,RT-PCR,克隆,序列分析

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Abstract:

BMP-15基因主要在哺乳动物卵巢中表达,对卵泡的发育和分化起重要作用,克隆了牛BMP-15成熟肽编码区的cDNA序列并进行序列分析,旨在为BMP-15在牛繁殖性能方面的进一步研究奠定理论基础。根据其他物种BMP-15基因的保守序列设计特异性引物,扩增牛的cDNA序列。从牛卵巢中提取总RNA,采用RT-PCR技术扩增出牛BMP-15cDNA序列。将此片段克隆到PMD18-T载体中,经菌落PCR鉴定和DNA序列测定分析验证,证实所克隆序列BMP-15为骨形态发生蛋白,符合骨形态发生蛋白基因的特征。序列分析表明,该cDNA包含有1185bp组成的开放读码框(ORF),该ORF编码39个氨基酸,与绵羊、猪、人、小鼠等动物氨基酸序列比对发现同源性分别为98.5%,87.6%,7.0%,69.%。

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