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鸡MuslincDNA克隆与序列分析

DOI: 10.7668/hbnxb.2007.04.013, PP. 56-58

Keywords: ,Musclin,克隆,序列分析

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Abstract:

基于电子延伸序列,本试验克隆并分析了鸡Muslin基因。肌肉组织提取总RNA,利用设计的引物进行RT-PCR,PCR产物与pMD19-T连接后转化E。coliDH5α,检测阳性克隆并测序;克隆的Musclin基因与人、大鼠、小鼠同源性分别为74%,7%,70%,推测的氨基酸序列同源性分别为58%,54%,50%,氨基酸序列含有“KKKR”结构和与小鼠ANP,BNP,CNP蛋白的同源性区域。试验成功克隆了鸡Musclin基因并注册GenBank(EF551041)。

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