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夹竹桃苯丙氨酸解氨酶的基因克隆与序列分析

DOI: 10.7668/hbnxb.2007.04.005, PP. 19-24

Keywords: NoPAL1,苯丙氨酸解氨酶,夹竹桃,基因克隆,序列分析

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Abstract:

首次从夹竹桃中克隆得到苯丙氨酸解氨酶(PAL)基因cDNA片段,并命名为NoPAL1,GenBank登录号为AY74777,这也是首次从夹竹桃花青苷代谢途径中克隆得到的基因。NoPAL1长8个bp,编码289个氨基酸。通过核苷酸和蛋白质序列多重比较发现NoPAL1与其他植物的PAL基因高度同源。NoPAL1编码的蛋白质序列包含与水稻、玉米PAL蛋白质相同的脱氨基位点和催化活性位点。PAL系统进化树表明,NoPAL1与乔木类植物的PAL基因聚类关系最近。克隆NoPAL1基因为利用基因工程技术来调控夹竹桃花青苷的代谢从而调控夹竹桃花的颜色提供了基因资源。

References

[1]  Campos R, Nonogaki H, Suslow T, et al. Isolation and characterization of a wound inducible pheylalanine ammonia-lyase gene(LsPAL1) from Romaine lettuce leaves[J]. Physiol Plant, 2004, 121: 429-438.
[2]  Lois R, Dietrich A, Hahlbrock K, et al. A phenylalanine ammonia-lyase gene from parsley structure, regulation and identification of elicitor and light responsive cis-acting elements[J]. EMBO J, 1989, 8: 1641-1648.
[3]  Ohl S, Hedrick S A, Chory J, et al. Functional properties of a phenylalanine ammonia-lyase promoter from Arabidopsis[J]. Plant Cell, 1990, 2: 837-848.
[4]  Lee S W, Robb J, Nazar R N. Truncated phenylalanine ammonia-lyase expression in tomato(Lycopersicum esculentum)[J]. J Biol Chem, 1992, 267: 11824-11830.
[5]  Zhu Q, Dabi T, Beeche A, et al. Cloning and properties of a rice gene encoding phenylalanine ammonia-lyase[J]. Plant Mol Biol, 1995, 29: 535-550.
[6]  Joos H J, Hahlbrock K. Phenylalanine ammonia-lyase in potato(Solanum tuberosum L. ). Genomic complexity, structural comparison of two selected genes and modes of expression[J]. FEBS, 1992, 204: 621-629.
[7]  Liang X, Dron M, Cramer C L, et al. Differential regulation of phenylalanine ammonia-lyase genes during plant development and by environmental cues[J]. J Biol Chem, 1989, 264: 14486-14492.
[8]  Ding B Y. The environmental protective plant Nerium oleander[J]. Technology and Market, 2004, 9: 37 (in Chinese).
[9]  Johnson W. A simple and efficient Method for Isolating RNA from pine tree[J]. Plant Physiol, 1997, 113(1): 176-179.
[10]  Kumar S, Tamura K, Nei M. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment[J]. Briefings In Bioinformatics, 2004, 5: 150-163.
[11]  Alokam S, Li Y, Li W, et al. Photoregulation of phenylalanine ammonia-lyase(PAL) and chalcone synthase (CHS) in the accumulation of anthocyanin in alpine and prairie ecotypes of Stellaria longipes under varied R/FR[J]. Physiol Plant, 2002, 116: 531-538.
[12]  Nakazawa A, Nozue M, Yasuda H. Expression pattern and gene structure of phenylalanine ammonia-lyase in Pharbitis nil[J]. J Plant Res, 2001, 114: 323-328.
[13]  Kumar A, Ellis B. The phenylalanine ammonia-lyase gene family in raspberry structure, expression, and evolution[J]. Plant Physiol, 2001, 127: 230-239.
[14]  Kostenyuk I A, Zon J, Burns J K. Phenylanine ammonia lyase gene expression during abscission in citrus[J]. Physiol Plant, 2002, 116: 106-112.
[15]  Lafuente M T, Zacarias L, Martinez-Tellez M A, et al. Phenylalanine ammonia-lyase and ethylene in relation to chilling injury as affected by fruit age in citrus[J]. Postharvest Biol Tech, 2003, 29: 308-317.
[16]  Kuhn D N, Chappell J, Boudet A, et al. Induction of phenylalanine ammonia-lyase and 4-coumarates: CoA ligase mRNAs in cultured plant cells by UV light or fungal elicitor[J]. Proc Natl Acad Sci USA, 1984, 81: 1102-1106.
[17]  Leyva A, Jarillo J A, Salinas J, et al. Low temperature induces the accumulation of phenylalanine ammonia-lyase and chalcone synthase mRNAs of Arabidpsis thaliana in a light-dependent manner[J]. Plant Physiol, 1995, 108: 39-46.
[18]  Cramer C L, Edwards K, Dron M, et al. Phenylalanine ammonialyase gene organization and structure[J]. Plant Mol Biol, 1989, 12: 367-383.
[19]  Minami E, Ozeki Y, Matsuoka M, et al. Structure and some characterization of the gene for phenylalanine ammonia-lyase from rice plants[J]. Eur J Biochem, 1989, 185: 19-25.
[20]  Subramaniam R, Reinold S, Molitor E K, et al. Structure, inheritance, and expression of hybrid poplar (Populus trichocarpa Populus deltoids) phenylalanine ammonia-lyase genes[J]. Plant Physiol, 1993, 102: 71-83.
[21]  Fukasawa-Akada T, Kung S D, Watson J C. Phenylalanine ammonia-lyase gene structure, expression, and evolution in Nicotiana[J]. Plant Mol Biol, 1996, 30: 711-722.
[22]  Whetten R W, Sederoff R R. Phenylalanine ammonia-lyase from loblolly pine Purification of the enzyme and insolation of complementary DNA clones[J]. Plant Physiol, 1992, 98: 380-386.
[23]  Mao Y Q, Li N. The exploiture and utilization of Nerium oleander[J]. Special Economic Species of Animal and Plant, 2005, 3: 36-37 (in Chinese).
[24]  Peng X W, Yang L Y, Chen Y W, et al. Study on the antibacterial activity of endophytic fungi isolated from Thevetia peruviana[J]. J Fungi Res, 2003, 1 (1): 33-36(in Chinese).
[25]  Tewtrakul S. Nakamura N, Hattori M, et al. Flavanone and flavonol glycosides from the leaves of Thevetia peruviana and their HIV-1 reverse transcriptase and HIV-1 integrase inhibitory activies[J]. Chem Pharm Bull, 2002, 50 (5): 630-635.
[26]  Rosler J, Krekel F, Amrhein N, et al. Maize phenylalanine ammonia-lyase has tyrosine ammonia-lyase activity[J]. Plant Physiol, 1997, 113 (1): 175-179.
[27]  Paul A, Tony A, Jeremy J. Characterization of a phenylalanine ammonia-lyase multigene family in Trifolium subterraneum[J]. Gene, 1994, 138(1): 87-92.
[28]  Holton T A, Cornish E C. Genetics and biochemistry of anthocyanin biosynthesis[J]. Plant Cell, 1995, 7: 1071-1083.
[29]  van der Meer I M, Stuitje A R, Mol J N M. Regulation of general phenylpropanoid and flavonoid gene expression[C]//Verma DPS. Control of Plant Gene Expression Boca Raton, FL, CRC Press, 1993: 125-155.

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