OALib Journal期刊
水稻谷蛋白GluB-4基因启动子克隆及载体构建
DOI: 10.3321/j.issn:1000-7091.2007.02.006
Keywords: 水稻 ,GluB-4基因 ,启动子 ,谷蛋白
Abstract:
GluB-4是水稻种子谷蛋白的编码基因，在种子成熟过程中，由GluB-4启动子调控，在胚乳中特异地表达。以水稻基因组DNA为模板，通过PCR扩增技术得到GluB-4启动子片段，序列分析结果表明：获得的启动子片段的大小为1560bp，与已报道的该启动子序列相比较，其核苷酸序列同源性为99.8%。该启动子区域含有TATA-box，CAAT-box，GCN4基序，Skn-1基序等胚乳特异表达启动子所必需的正调控元件，同时还含有高水平转录调控因子5UTRPy-richstretch序列。利用该启动子构建了植物种子特异表达载体pCGB4P，为进一步的研究工作奠定了基础。
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