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产耐热直接相关溶血素的副溶血性弧菌PCR检测及其表型分析

DOI: 10.7668/hbnxb.2008.S2.025, PP. 114-117

Keywords: 副溶血性弧菌,耐热直接相关溶血素,PCR,尿素酶

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Abstract:

运用PCR技术成功建立了检测产耐热直接相关溶血素(TRH)的副溶血性弧菌的方法,并应用该方法从1株标准株和其他3种不同来源的37株副溶血性弧菌中的13株扩增出0.5kb的trh基因特异性扩增带;产TRH的副溶血性弧菌中1株标准株,12株分离自腹泻病人排泄物或呕吐物,1株分离自水产动物养殖用水;并且产TRH与尿素酶阳性的副溶血性弧菌菌株之间的具有一一对应的关系,结果认定尿素酶阳性是产TRH的副溶血性弧菌菌株的表现型。

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