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pCDNA3-CT真核表达质粒的构建及其在COS-1细胞中的表达

DOI: 10.7668/hbnxb.2008.S1.004, PP. 15-18

Keywords: 甲状旁腺素,转染,IFA

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Abstract:

通过酶切方法自含鸡甲状旁腺素(CT)基因的克隆质粒pGEM-T-CT中扩增CT基因,并将其克隆到真核表达载体pCDNA3中,阳性克隆鉴定后,在PEI作用下转染COS-1细胞,通过间接免疫荧光试验(IFA)检测到了CT在COS-1中的表达。

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