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绵羊Gastorkine-1基因的克隆与序列分析

DOI: 10.7668/hbnxb.2008.01.009, PP. 38-40

Keywords: 绵羊,胃黏膜保护因子,克隆,序列分析

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Abstract:

基于电子延伸序列,克隆并分析了绵羊Gastorkine-1基因。提取皱胃黏膜组织总RNA,利用设计的引物进行RT-PCR,PCR产物与pMD19-T载体连接后转化E.coliJM109感受态细胞,检测阳性克隆并测序。克隆的绵羊Gas-torkine-1基因长619bp,编码185个氨基酸,与人、小鼠、猪、牛的同源性分别为82.0%,48.8%,85.4%,96.9%,推测的氨基酸序列信号肽为1~20aa,BRICHOS结构域为54~150aa,结构特征与人、小鼠、猪、牛的Gastorkine-1相一致。

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