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人β淀粉样蛋白基因植物表达载体的构建及转化

DOI: 10.7668/hbnxb.2009.S1.006, PP. 23-26

Keywords: &beta,淀粉样蛋白,植物表达载体,转基因烟草

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Abstract:

人β淀粉样蛋白(Amyloidβpeptide,Aβ)是治疗阿尔茨海默氏病(Alzheimer′sdisease,AD)的关键靶标之一。本试验利用重叠PCR技术获得2Aβ串联基因片段,并将其克隆到植物表达载体pBIDST(含双35S启动子和TEV增强子)中。用根癌农杆菌EHA105介导转化烟草,筛选获得了卡那霉素抗性植株。PCR和RT-PCR检测结果证实2Aβ基因已经整合进烟草基因组中并进行了转录。

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