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小麦和水稻auxin基因家族的生物信息学比较分析

DOI: 10.7668/hbnxb.2009.06.004, PP. 15-19

Keywords: 小麦,水稻,植物生长素

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Abstract:

根据测序获得的1条260bpcDNA片段,通过预测发现其包含小麦植物生长素(AUXIN)基因的部分编码序列,通过电子延伸、设计引物,从小麦Mardler/7*百农3217的cDNA中扩增获得一条608bp的cDNA片段,该基因序列数据库(GenBank)登录号为AY902381(基因)和(蛋白).编码202个氨基酸,预计蛋白的分子量为23.0kDa,等电点为9.93.利用已经分离的小麦生长素(AUXIN)基因的保守序列为检索序列,对小麦和水稻中的AUXIN基因家族成员进行分析,利用这些基因编码蛋白序列构建系统发生树,查找在GenBank的EST数据库中查找这些基因的ESTs表达序列,分析了这些基因在细胞中的定位情况和蛋白结构的相似性,根据已知相似基因的功能,分析该基因有进一步深入研究的必要.

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