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花生野生近缘种生物素羧基载体蛋白基因的克隆与结构分析

DOI: 10.7668/hbnxb.2009.06.002, PP. 6-10

Keywords: 花生,生物素羧基载体蛋白,基因克隆,基因结构

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Abstract:

根据栽培花生鲁花14生物素羧基载体蛋白基因accB1和accB2cDNA序列设计基因特异引物,克隆了花生野生近缘种Arachisduranensis、A.rigonii、A.batizocoi和A.hoehnei的accB1和accB2cDNA序列.序列分析表明,accB1和accB2在栽培花生和野生近缘种中高度保守,氨基酸序列一致性分别高于98.6%和97.%.A基因组A.duranensis、A.rigonii和B基因组A.batizocoi、A.hoehnei的accB1和accB2的氨基酸序列存在差异,但与基因组来源无关.花生野生近缘种A.duranensis、A.rigonii、A.batizocoi和A.hoehneiaccB2基因组序列分析表明,栽培花生和野生近缘种的accB2的基因结构相同.A基因组的A.duranensis与B基因组的A.batizocoi和A.hoehnei的accB2基因组DNA核苷酸序列一致性分别为98.9%,98.8%,而A基因组的A.rigonii与A.duranensis、A.batizocoi和A.hoehnei的序列一致性分别为93.%,93.6%,93.4%.研究表明,生物素羧基载体蛋白基因在栽培花生及野生近缘种中非常保守.

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