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早熟油桃紫金红1号亲本的SSR鉴定

DOI: 10.7668/hbnxb.2009.06.042, PP. 205-209

Keywords: 油桃,SSR,亲本鉴定

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Abstract:

油桃新品种紫金红1号是从早熟油桃中实生选育的,果实经济性状优良.以23个疑似亲本的油桃品种为试材,利用SSR标记共显性的特点,进行了紫金红1号亲本鉴定.SSR-PCR扩增结果表明,163对SSR引物中有48对能在试材中扩增出多态性良好、谱带清晰的产物,其中17对SSR引物扩增出的20个SSR位点呈现共显性带型.基于18个SSR等位基因信息的亲缘关系分析发现,紫金红1号与早红2号之间的亲缘关系最近,相似系数为0.881,在树状图上也聚为一类.经共显性SSR位点遗传规律分析,使用3个SSR引物(CPPCT1、BPPCT031、UDP96-018)的扩增位点,可排除早红2号以外的其他品种作为紫金红1号亲本的可能.认为早红2号自交获得紫金红1号可能性很大,或至少是其亲本之一.

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