全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

C型口蹄疫病毒衣壳蛋白前体P1基因的原核表达及其生物活性的初步分析

DOI: 10.7668/hbnxb.2009.05.002, PP. 7-10

Keywords: C型口蹄疫病毒,重组蛋白P1,表达生物活性

Full-Text   Cite this paper   Add to My Lib

Abstract:

将口蹄疫病毒(FMDV)结构蛋白基因P1的完整cDNA序列插入原核表达性载体pET-28α(+)中,获得融合表达质粒pET-P1,转化E.coli.BL21(DE3),经IPTG诱导,SDS-PAGE结果表明,pET-P1获得融合表达,WesternBlot检测证实表达的融合蛋白具有免疫活性,表达产物主要以包涵体的形式存在,进一步采用纯化试剂盒纯化P1蛋白做为诊断抗原。

References

[1]  Saiz M,Nune J I,Jimenez M.Foot-and-mouth disease virus biology and prospects for disease control[J].Microbes Infect,2002,4(1):1103-1192.
[2]  Ling Jie-yu,Liu Zhao,Tong Tie-zhu.Construction and eukaryotic expression of recombinant plasmid encoding fusion protein of goat complement C3d and foot and mouth disease virus VP1[J].Chinese Journal of Biotechnology,2008,24(2):209-213.
[3]  周建华,丛国正,高闪电.口蹄疫病毒株OA/58L蛋白酶的结构构建和功能分析[J].华北农学报,2007,22(4):176-179.
[4]  Li Yan-min,Catrina M A,Stirling Michael S Denyer.Dramatic improvement in FMD DNA vaccine efficacy and cross-serotype antibody induction in pigs following a protein boost[J].Vaccine,2008,26:2647-2656.
[5]  Balamurugan V,Renji R,Ana S N,et al.Protective immune response of the capsid prectorsor polypetide(P1) of foot and mouth disease virus type O produced in pichina postors[J].Vaccine,2003,92:141-149.
[6]  潘丽,张永光,王永录,等.口蹄疫结构蛋白P1-2A及蛋白酶3C基因的转基因番茄对豚鼠免疫反应的初步研究[J].病毒学报,2006,22(4):297-303.
[7]  Sanz Parra A,Jimenez Clavero M A,Garc Briones M M,et al.Recombinant viruse expression the foot-and-mouth disease virus capsid precursur polypetide P1 induce celluiar or not humoral antiviral immunity and partial protection in pigs[J].Virology,1999,259:129-134.
[8]  余晓岚,肖少波,方六荣,等.口蹄疫病毒P1基因在大肠杆菌中的高效表达及其生物活性的初步分析[J].生物工程学报,2005,21(1):163-167.
[9]  Sanz A,Vazquez B,Sobrino F,et al.Evidence of partial protection against foot-and-mouth disease in cattle immunized with a recombinant adenovirus vector expressing the precursor polyeptide(P1)of foot-and-mouth disease virus capsid protein[J].Gen Virol,1999,80(3):671-679.
[10]  周建华,丛国正,高闪电,等.口蹄疫OA/58病毒株CP2蛋白结构模拟与B细肥抗原原表位的分析[J].华北农学报,2008,23(3):5-8.
[11]  Berinstein A,Taboga,Smitsaart E,et al.Protective immunity against foot-and-mouth disease viruse induced by a recombint vaccinia virus[J].Vaccine,2000,18:2231-2238.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133