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大豆炭腐病病原菌鉴定

DOI: 10.7668/hbnxb.2009.05.040, PP. 192-196

Keywords: 大豆,炭腐病,菜豆壳球孢菌,鉴定

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Abstract:

为有效防冶大豆炭腐病,对最近在北京和天津两块大豆田发生的疑似大豆炭腐病病原菌进行了鉴定。在PDA培养基病原菌上分离物生长较快,菌落灰色,产生大量直径为49.8~111.4μm的黑色菌核。接种表明,选择的5个分离物对大豆品种合丰25的幼苗致病,且各分离物从病株上重新分离率为100%。用通用引物ITS4/ITS5扩增5个分离物的rDNA-ITS序列区和测序,通过与Genbank数据库比对,5个分离物与菜豆壳球孢菌分离物序列的相似性为97%~99%。用菜豆壳球孢菌的特异性引物MpKF1/MPKR1进行检测,在5个分离物中均扩增出350bp的特征片段。基于病害症状、病原菌形态、致病性和分子特征,鉴定这5个分离物为菜豆壳球孢菌。该病害在华北地区尚属首次报道。

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