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应用乙肝核心抗原增强肌肉抑制素免疫原性的研究

DOI: 10.7668/hbnxb.2009.05.041, PP. 197-200

Keywords: 肌肉抑制素,乙肝核心抗原,融合蛋白,免疫原性

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Abstract:

为提高肌肉抑制素(Myostatin,MSTN)免疫原性,进行了乙肝核心抗原(HBcAg)作为分子佐剂可能性的研究。利用PCR方法获得了MSTN的C端片段,并分别在乙肝核心抗原的N端、C端及中间位点融合,构建了3个融合表达克隆:pET-30a-MSTN/HBcAg(N)、pET-30a-MSTN/HBcAg(C)和pET-30a-MSTN/HBcAg(M),转化大肠杆菌后IPTG诱导表达,然后利用Ni2+亲和层析纯化融合蛋白。应用重组蛋白免疫小鼠后利用间接ELISA法检测抗血清效价。结果表明:融合蛋白免疫效果要显著优于MSTN(P<0.05);融合蛋白MSTN/HBcAg(M)的免疫效果最佳。乙肝核心抗原可以作为分子佐剂以增强肌肉抑制素免疫原性。

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