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缺磷胁迫对黑籽南瓜幼苗根系生长和根系分泌物的影响

DOI: 10.7668/hbnxb.2009.05.034, PP. 164-169

Keywords: 缺磷,黑籽南瓜,根系生长,根系分泌物

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Abstract:

采用营养液培养方法,以1/2单位日本园试配方为基本营养液,在温室内培养黑籽南瓜(CucurbitaficifoliaB.)幼苗,测定正常供磷处理和缺磷胁迫下黑籽南瓜幼苗在根系生长、根系形态和根系分泌物等方面的差异,以期为南瓜磷高效种质创新和耐低磷机制研究提供理论依据。结果表明,缺磷胁迫后21d,黑籽南瓜幼苗地上部生物量显著降低,根系生物量与正常供磷处理相比没有明显差异,根冠比增大;总根长和根表面积显著增加,根数、根横径和根体积与正常供磷处理相比没有明显的差异;根系吸磷量和磷转运率下降,根系吸磷量占总吸磷量的百分数增加,根系磷利用效率显著增高。总根长和根表面积增加可能与其磷吸收效率密切相关,是黑籽南瓜幼苗对缺磷胁迫的适应性变化。与正常供磷处理相比,缺磷胁迫后21d,黑籽南瓜幼苗根系分泌的化合物种类组成类似,主要由酚类、芳香烃类、酯类、胺类、烯烃类和烷烃类化合物组成。但缺磷胁迫后根系分泌物中的化学成分多于正常供磷处理,邻苯二甲酸二(1丁基2异丁基)酯等8种化合物是缺磷胁迫下黑籽南瓜根系分泌物的特有成分。缺磷胁迫后黑籽南瓜的自毒作用增强。

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