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牦牛SRY基因克隆与分子特征

DOI: 10.7668/hbnxb.2009.04.011, PP. 50-56

Keywords: 牦牛,SRY基因,克隆,分子特征

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Abstract:

SRY(Sex-determiningregionontheYchromosome,SRY)基因位于哺乳动物的Y染色体,对性别形成起着决定性作用.对高原牦牛SRY基因的编码区进行克隆和分子特征分析,以期从分子水平了解牦牛的性别形成机制.以雄性高原牦牛血液为材料,从基因组DNA中扩增SRY基因编码区(单外显子)序列,将其克隆至pGEM-Teasy载体并测序.同时,将牦牛SRY基因编码区与奶牛进行序列比对;对牦牛SRY蛋白与其他物种SRY蛋白进行序列比对;采用在线生物软件对牦牛SRY蛋白的特性和结构进行预测.牦牛SRY基因(GenBank:EU5425)编码区长68bp,编码229个氨基酸.克隆获得的牦牛SRY基因编码区与奶牛该序列存在2个碱基的变异,造成1个氨基酸的变异;各物种SRY蛋白具有较高的同源性;牦牛SRY蛋白(GenBank:ACB2999)主要由亲水性氨基酸构成,同源建模预测的SRYHMG区域的3D模型显示,SRYHMG区域三维结构呈由三个α-螺旋组成的"L"型.首次从高原牦牛基因组中克隆了SRY基因,并进一步揭示了其分子特征,为从分子水平人为的控制牦牛性别奠定了重要基础.

References

[1]  Wiederstein M,Sippl M J.ProSA-web:interactive web service for the recognition of errors in three-dimensional structures of proteins[J].Nucleic Acids Research,2007,35:407-410.
[2]  陈秋生,冯霞,姜生成.牦牛肺脏高原适应性的结构研究[J].中国农业科学,2006,39(10):2107-2113.
[3]  Sinclair A H,Berta P,Palmer M S,et al.A gene from the human sex-determining region encodes a protein with homology to conserved DNA-binding motif[J].Nature990,346:240-244.
[4]  Pannetier M,Tilly G,Kocer A,et al.Goat SRY induces testis development in XX transgenic mice[J].FEBS Letters,2006,580(15):3715-3720.
[5]  Shahid M,Dhillon V S,Aslam M,et al.Three new novel point mutations localized within and downstream of high-mobility group-box region in SRY gene in three indian females with turner syndrome[J].The Journal of Clinical Endocrinology & Metabolism,2005,90(4):2429-2435.
[6]  Li B,Phillips N B,Jancso-Radek A,et al.SRY-directed DNA bending and human sex reversal:reassessment of a clinical mutation uncovers a global coupling between the HMG box and its tail[J].Journal of Molecular Biology,2006,360(2):310-328.
[7]  Yoshiakira K,Ryuji H,Shogo M,et al.From SRY to SOX9:mammalian testis differentiation[J].Jounal of Biochemistry,200538(1):13-19.
[8]  Barrionuevo F,Bagheri-Fam S,Klattig J,et al.Homozygous inactivation of Sox9 causes complete XY sex reversal in mice[J].Biology of Reproduction,2006,74:195-201.
[9]  白文林,尹荣焕,罗光彬,等.辽宁绒山羊SRY基因编码区的分子特征研究[J].华北农学报,2008,23(5):44-48.
[10]  J 萨姆布鲁克,D W拉塞尔.分子克隆实验指南[M].第3版.北京:科学出版社,2002:27-30.
[11]  Kyte J,Doolittle R F.A simple method for displaying the hydropathic character of a protein[J].Journal of Molecular Biology98257(1):105-132.
[12]  Garnier J,Osguthorpe D J,Robson B.Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins[J].Journal of Molecular Biology97820(1):97-120.
[13]  Lambert C,Leonard N,De Bolle X,et al:Prediction of proteins 3D structures[J].Bioinformatics,20028(9):1250-1256.
[14]  Wilhelm D,Palmer S,Koopman P.Sex determination and gonadal development in mammals[J].Physiological Reviews,2007,87:1-28.
[15]  Ishii M,Tachiwana T,Hoshino A,et al.Potency of testicular somatic environment to support spermatogenesis in XX/Sry transgenic male mice[J].Development,200734:449-454.
[16]  Modi D,Shah C,Sachdeva G,et al.Ontogeny and cellular localization of SRY transcripts in the human testes and its detection in spermatozoa[J].Reproduction,200530:603-613.
[17]  Biaoru L,Wei Z,Ging C,et al.Human sex reversal due to impaired nuclear localization of SRY[J].Jounal of Biochemistry,2001,276(49):46480-46484.
[18]  Harley V R,Arkson M J,Gentaro A.The molecular action and regulation of the testis determining factors,SRY and Sox9[J].Endocrine Reviews,2003,24(4):466-487.
[19]  Pohler J R,Norman D G,Bramham J,et al.HMG box proteins bind to four-way DNA junctions in their open conformarion[J].The EMBO Journal9987(3):817-826.
[20]  Leo D,Blanche C.SRY and the standoff in sex determination[J].Molecular Endocrinology,2008,22(1):1-9.
[21]  Colman P M.Structure-based drug design[J].Current Opinion in Structural Biology994,4:868-874.
[22]  Johnson M S,Srinivasan N,Sowdhaminiand R et al.Knowledge-based protein modeling[J].Critical Reviews in Biochemistry and Molecular Biology994,29:1-68.

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