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虹鳟鱼NDK-1基因cDNA的克隆与序列特征分析

DOI: 10.7668/hbnxb.2009.04.014, PP. 70-73

Keywords: 虹鳟鱼,NDK-1基因,RT-PCR,RACE

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Abstract:

以虹鳟鱼(Oncorhynchusmykiss)为研究材料,使用RT-PCR和RACE法分离和克隆了虹鳟鱼NDK-1基因cDNA的全长序列(GenBank登录号:(FJ607868).序列全长953bp,其中5'端非翻译区(5'-UTR)长5bp,3'端非翻译区(3'-UTR)长28bp,开放性阅读框(ORF)长62bp,编码207个氨基酸.虹鳟鱼NDPK蛋白序列与几种脊椎动物台湾樱花钩吻鲑、斑马鱼、西方爪蟾、非洲爪蟾、人和黑青斑河的同源性分别为95%,72%,68%,68%,65%和6%,表明NDK-1基因在进化过程中是高度保守的.

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