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牛肌肉生长抑制素基因编码区全序列的分子进化特征

DOI: 10.7668/hbnxb.2009.04.010, PP. 46-49

Keywords: 牛种,肌肉生长抑制素基因,编码区序列,分子进化

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Abstract:

运用PCR分段扩增测序法,获得普通牛、瘤牛、牦牛、大额牛、水牛(沼泽型和河流型)MSTN基因编码区全序列,进而分析编码区序列的分子进化特征.结果显示,牛MSTN基因编码区全序列长1128bp,种内核苷酸突变率为0~0.35%,种间核苷酸突变率为0.08%~1.2%.MSTN基因编码区序列存在密码子使用偏好性,29个偏好性密码子约占密码子使用总数的9.2%.核苷酸的替代以转换为主,转换/颠换比为2.9.非同义突变位点远少于同义突变位点,同义与非同义替代速率比全部小于或等于1,表明MSTN基因编码区序列并未受到达尔文正向选择的影响,却受到一定的负向选择作用.分子进化树显示,水牛、瘤牛独自成类并与其他牛种显著分化;普通牛、牦牛、大额牛间的序列分化程度并不明显,但有可能在进化过程中逐步产生明显的分化.

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