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诱导型和组成型表达的冷诱导转录因子CBF1基因表达载体的构建

DOI: 10.7668/hbnxb.2009.03.009, PP. 41-45

Keywords: 拟南芥,CBF1,CBF1启动子,表达载体构建

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Abstract:

为研究冷诱导转录因子CBF1(C-repeat-binding-factor)基因对我国优良豆科牧草--苜蓿的抗寒性的改良作用,试验从拟南芥(Arabidopsisthaliana)中利用PCR方法分离了CBFI基因及其启动子,分别构建了CaMV3S启动子和来源于拟南芥的CBFI启动子驱动的农杆菌介导的植物转化表达载体,为下一步研究利用CBF1基因改良苜蓿抗逆性奠定了基础.

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