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一种紫色大白菜细胞质不育系的分子鉴定

DOI: 10.7668/hbnxb.2009.03.037, PP. 174-178

Keywords: 紫色大白菜,OguCMS,orF138,应用

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Abstract:

为了获得紫色大白菜细胞质不育系的特异序列,鉴定该不育系所属的不育类型,应用设计的orF138上、下游引物PCR扩增6份材料:紫色大白菜CMS不育系,保持系07-721,杂交F1,3个回交BC1单株BC1-1、BC1-2和BC1-3.对获得的差异片段进行克隆、测序,同源性比较.结果表明,在紫色大白菜CMS不育系、杂交F1、3个回交BC1单株上均扩增到309bp的特异产物,而保持系07-721没有扩增产物.测序和同源性比对结果表明,紫色大白菜CMS的orF138与萝卜Ogura不育系的orF138、芸薹属作物与萝卜OguCMS体细胞杂种的orF138同源性均达到99%,E值为2e-1.证明了紫色大白菜CMS不育系为一种改良的Ogura萝卜细胞质不育系,同时初步探讨了该不育系和紫色基因的应用前景.

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