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西瓜抗枯萎病相关EST-SSR的信息分析

DOI: 10.7668/hbnxb.2009.03.019, PP. 87-91

Keywords: 西瓜,枯萎病,EST,SSR

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Abstract:

对西瓜枯萎病菌生理小种1诱导的抑制差减杂交cDNA文库测序获得的4000条EST进行分析,经过前处理后拼接得到1487条unigene序列,全长为79kb.在其中978条unisene序列中共检索出2136个EST-SSR,出现频率为3.4%.EST-SSR的平均分布距离和平均长度分别是1/0.36kb、19.9bp.EST-SSRs中的重复单元以二核苷酸和三核苷酸重复为主,二者在总EST-SSRs中的出现频率为98.08%.GA/TC和GAA/TTC是二、三核苷酸中的优势重复类型,分别占二、三核苷酸重复的2.78%和12.97%.西瓜抗枯萎病相关EST资源的SSR信息分析为进一步建立西瓜EST-SSR标记和探索其在西瓜基因组学研究中的应用奠定了基础.

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