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灰飞虱体内沃尔巴克氏体的检测

DOI: 10.7668/hbnxb.2009.01.039, PP. 178-180

Keywords: 灰飞虱,沃尔巴克氏体,检测

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Abstract:

采用沃尔巴克氏体(Wolbachia)表面蛋白基因(wsp基因)的通用引物81F和691R对河北曲阳和容城灰飞虱(Laodelphaxstriatellus)体内的Wolbachia进行PCR检测,结果表明,从灰飞虱DNA样品中可扩增出600bp大小的wsp目的基因片段,证实河北曲阳和容城田间的灰飞虱种群有Wolbachia感染对wsp目的基因片段的序列测定和同源性分析表明,河北的2个灰飞虱种群感染的Wolbachia与来自白背飞虱的wFur品系、灰飞虱的wStri品系亲缘关系较近,同属于WolbachiaB大组Con组。

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