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大白菜DREB类转录因子cDNA的克隆及植物表达载体的构建

DOI: 10.7668/hbnxb.2009.01.016, PP. 69-73

Keywords: 大白菜,DREB转录因子,基因克隆,植物表达载体

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Abstract:

以干旱处理的大白菜自交系85-1为材料,利用RT-PCR技术获得了大白菜DREB类转录因子基因的cDNA编码序列,命名为BcDREB1(GenBank登录号为:EU924266)。序列分析表明,BcDREB1核苷酸序列长663bp,编码214个氨基酸,含有一个典型的AP2结构域,具有DREB转录因子的典型特征。将该基因的cDNA编码序列连接到植物表达载体pCAMBIA2301中,成功构建了大白菜BcDREB1基因的植物表达载体pCAM-BcDREB1,为进一步通过转基因技术研究该基因的功能奠定了基础。

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