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抗戊唑醇禾谷镰孢菌对渗透压敏感性及电导率变化研究

DOI: 10.7668/hbnxb.2011.S2.039, PP. 184-189

Keywords: 戊唑醇,禾谷镰孢菌,渗透压,电导率

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Abstract:

通过紫外诱导和药剂驯化获取禾谷镰孢菌的抗戊唑醇菌株,探索了抗戊唑醇禾谷镰孢菌的抗药性机制。抗药菌株经转接培养17代后筛选遗传稳定性抗性菌株,利用渗透压法和电导率法测定抗药菌株与亲本菌株之间的生理性差异。所获得的9个抗性菌株,抗性水平最大值为186.03,最小值为34.26。其中,有5个抗性菌株能保持较高的抗性水平。在含2.0%和4.0%的浓度葡萄糖培养基下,抗性菌株的菌落生长直径要比亲本菌株大,而含相同浓度的KCl培养基中,抗性菌株与敏感菌株的菌落生长变化无规律。在不同时间下,抗性菌株和亲本菌株的电导率变化明显,药剂驯化抗性菌株电导率变化比敏感菌株大,经200min后药剂驯化的抗性菌株比紫外诱导的抗性菌株电导率普遍高。禾谷镰孢菌抗戊唑醇的机制可能与细胞膜通透性的改变和短时间内快速排泄杀菌剂有关。

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