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重组HSP70对罗非鱼外周血淋巴细胞免疫功能的影响

DOI: 10.7668/hbnxb.2011.S2.045, PP. 213-218

Keywords: 热休克蛋白70,淋巴细胞,免疫功能,海豚链球菌,疫苗

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Abstract:

利用酵母重组分泌表达的罗非鱼HSP70与海豚链球菌抗原在体外非共价结合形成HSP70-抗原复合物,通过体外增殖试验及免疫相关基因表达检测研究HSP70对罗非鱼外周血淋巴细胞免疫功能的影响。结果表明,tHsp70在体外能够与海豚链球菌菌体、胞外分泌物(ECP)结合形成HSP70-抗原复合物。tHsp70(10μg/mL)或tHsp70(10μg/mL)-菌体复合物均可极显著刺激罗非鱼淋巴细胞增殖(P<0.01),而ECP对淋巴细胞增殖有一定的抑制作用(P=0.056),但tHsp70(10μg/mL)与ECP体外非共价结合可减轻ECP对细胞增殖的抑制作用。浓度为100μg/mL的tHsp70或tHsp70-抗原复合物作用于罗非鱼淋巴细胞,ICER、PGK、MMP9、MHCⅡ4个基因的表达量均极显著高于海豚链球菌抗原(菌体或ECP)单独刺激和无刺激对照组(P<0.01)。研究表明罗非鱼HSP70具有免疫增强和免疫佐剂的功能,为开发罗非鱼链球菌病HSP70-肽疫苗提供了试验依据。

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