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甘蓝幼苗叶片超微结构及细胞内Ca2+分布对低温的响应

DOI: 10.7668/hbnxb.2011.06.025, PP. 129-135

Keywords: 低温,甘蓝,超微结构,Ca2+分布

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Abstract:

探讨低温胁迫下甘蓝叶片细胞超微结构特征、细胞内Ca2+分布变化及品种间差异,为越冬甘蓝的耐寒性机理研究及耐寒品种选育提供理论依据。采用低温培养箱模拟逆境条件处理甘蓝幼苗,研究其对甘蓝叶片细胞超微结构及细胞内Ca2+分布的影响并比较了品种间差异。5℃低温处理时,甘蓝叶片大多数细胞器基本无损,排列有序,表明耐冷材料和冷敏材料均能适应5℃低温环境。在-3℃低温胁迫下,耐冷材料叶片超微结构破坏较小,而冷敏材料叶绿体结构破坏严重,基粒片层断裂、降解;线粒体多数扭曲变形,已无明显内脊。对照生长条件下(25/18℃),甘蓝幼苗叶肉细胞内Ca2+主要出现在液泡和细胞间隙中,细胞壁、细胞核中也有少量钙沉淀存在。在-3℃低温处理24h后,耐冷材料细胞质和细胞核中的Ca2+浓度明显增加,冷敏材料细胞质中Ca2+也有增加,但较耐冷材料少。在低温处理24~72h的过程中,耐冷材料细胞内增加的Ca2+浓度迅速降低,并恢复至处理前状态;而此阶段内冷敏材料因其细胞结构遭到严重破坏,细胞质中的Ca2+浓度升高到最大值后,无法恢复至稳定状态。低温胁迫下甘蓝叶片的超微结构特征及细胞内Ca2+行为特征表现显著差异,表明甘蓝叶片的超微结构特征及Ca2+分布变化与其抗寒性存在一定关系。

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