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大豆MYB基因GmMYBJ7的克隆及表达分析

DOI: 10.7668/hbnxb.2011.05.022, PP. 107-111

Keywords: 大豆,MYB转录因子,基因表达调控

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Abstract:

MYB蛋白是植物体中一类重要的转录因子,广泛参与植物生理代谢和发育过程的调节。本研究对利用RACE-PCR分离克隆的MYB转录因子基因GmMYBJ7的功能进行了初步研究。Norhern杂交对GmMYBJ7在不同组织中的表达情况进行了检测,结果只在茎、叶中检测到了GmMYBJ7的表达;酵母表达结果显示,GmMYBJ7具有明显的转录激活功能,β-半乳糖苷酶活性为24.31U;半定量RT-PCR检测显示,在表达GmMYBJ7的转化烟草中,类黄酮代谢途径中的苯丙氨酸氨基裂解酶(PAL)、肉桂酸-4-羟化酶(C4H)、4-香豆酰辅酶A连接酶(4CL)、查尔酮合成酶(CHS)、黄烷酮-3-羟化酶(F3H)等关键酶的表达显著降低,结果表明,GmMYBJ7可能参与植物类黄酮合成的调控。

References

[1]  杨文杰,杜海,方芳,等.大豆两个MYB转录因子基因的克隆与表达分析[J].中国农业科学,2008,41(4):961-970.
[2]  杨文杰,吴燕民,唐益雄.大豆转录因子基因GmMYBJ6的表达及功能分析[J].遗传,2009,31 (6):654-662.
[3]  杜海,杨文杰,刘蕾,等.大豆MYB转录因子基因GmMYBJ6和GmMYBJ7的克隆及表达分析[J].作物学报,2008,34(7):1179-1187.
[4]  Kranz H D,Denekamp M,Greco R,et al.Towards functional characterization of the members of the R2R3-MYB gene family from Arabidopsis thaliana[J].Plant J,1998,16:263-276.
[5]  Jin H,Martin C.Multifunctionality and diversity within the plant MYB gene family[J].Plant Mol Biol,1999,41:577-585.
[6]  Tamagnone L,Merida A,Parr A,et al.The AmMYB308and AmMYB330 transcription factors from Antirrhinum regulate Phenylpropanoid and lignin biosynthesis in transgenic tobacco[J].The Plant Cell,1998,10:135-154.
[7]  Jin H,Cominelli E,Bailey P,et al.Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis[J].EMBO J,2000,19:6150-6161.
[8]  Aharoni A,De Vos C H,Wein M,et al.The strawberry FaMYB1 transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco[J].Plant J,2001,28:319-332.
[9]  李雅轩,李蕊,孟凡臣,等.大豆吡哆醛激酶基因的克隆与表达分析[J].华北农学报,2009,24(3):27-31.
[10]  吴春霞.植物MBY基因研究进展[J].安徽农业科学,2009,20:70-72.
[11]  Riechmann J L,Heard J,Martin G,et al.Arabidopsis transcription factors:genome-wide comparative analysis among eukaryotes[J].Science,2000,290:2105-2110.
[12]  Martin C,Paz-Ares J.MYB transcription factors in plants[J].Trends Genet,1997,13:67-73.
[13]  Rabinowicz P D,Braun E L,Wolfe A D,et al.Maize R2R3-MYB genes:sequence analysis reveals amplification in the higher plants[J].Genetics,1999,153:427-444.
[14]  王栋,李利红,陈志玲,等.拟南芥根特异表达转录因子AtMYB305的鉴定及功能研究[J],科学通报,2001,46(21):1804-1809.
[15]  Carre I A,Kim J Y.MYB transcription factors in the Arabidopsis circadian clock[J].J Exp Bot,2002,53 (374):1551-1557.
[16]  Lee M M,Schiefelbein J.Developmentally distinct MYB genes encode functionally equivalent protein in Arabidopsis[J].Development,2001,128:1539-1546.
[17]  Bruce W,Folkerts O,Garnaat C,et al.Expression profiling of the maize flavonoid pathway genes controlled by estradiol-inducible transcription factors CRC and P[J].Plant Cell,2000,12:65-79.
[18]  Liu C J,Blount J W,Steele C L,et al.Bottlenecks for metabolic engineering of isoflavone glyconjugates in Arabidopsis[J].Proc Natl Acad Sci USA,2002,99:14578-14583.
[19]  Borevitz J O,Xia Y,Blount J,et al.Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis[J].Plant Cell,2000,12:2383-2394.
[20]  Nesi N,Jond C,Debeaujon I,et al.The Arabidopsis TT2gene encodes an R2R3 MYB domain protein that acts as a key determinant for proanthocyanidin accumulation in developing seed[J].Plant Cell,2001,13:2099-2114.
[21]  Mathews H,Clendennen S K,Caldwell C G,et al.Activation tagging in tomato identifies a transcriptional regulator of anthocyanin biosynthesis,modification and transport[J].The Plant Cell,2003,15:1689-1703.
[22]  Goodrich J,Carpenter R,Coen E S.Acommon gene regulates pigmentation pattern in diverse plant species[J].Cell,1992,68:955-964.
[23]  Chen Bo-Jun,Wang Yong,Hu Yuan-Lei,et al.Cloning and characterization of a drought-inducible MYB gene from Boca crassifolia[J].Plant Science,2005,168:493-500.
[24]  Liao Yong,Zou Hong-Feng,Wang Hui-Wen,et al.Soybean GmMYB76,GmMYB92,and GmMYB177 genes confer stress tolerance in transgenic Arabidopsis plants[J].Cell Research,2008,18:1047-1060.

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