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两株芽孢杆菌的鉴定及淀粉酶基因的克隆

DOI: 10.7668/hbnxb.2011.05.021, PP. 103-106

Keywords: 枯草芽孢杆菌,解淀粉芽孢杆菌,16SrDNA,淀粉酶基因

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Abstract:

对分离得到的两个菌株(B.H和B.M)进行鉴定,并对它们在芽孢杆菌培养基和淀粉富集培养基上的形态进行观察,通过对其16SrDNA序列的分析,构建系统进化树,确定B.H为枯草芽孢杆菌,B.M为解淀粉芽孢杆菌。根据已报道的枯草芽孢杆菌和解淀粉芽孢杆菌淀粉酶基因保守区域设计引物,分别以两个菌株的基因组DNA为模板,采用PCR扩增的方法成功获得两个淀粉酶基因,其编码区的长度分别为1434bp和1563bp,为该菌株的进一步利用开发提供了数据支持。

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